[166.692 Introduction to Microscopy in Biology] EXAM 02.02.2021

Here are the questions from the exam:

  1. How are non-conductive biological samples prepared for scanning electron microscopy (SEM)? Draw a scheme!
  2. Explain the principle of EFTEM (Energy Filtered Transmission Electron Microscopy. Draw a scheme!
  3. Explain the two methods of cryo fixation of biological samples.
  4. What are the intrinsic properties that characterize a fluorophore?
  5. Describe the technique of polarization microscopy.
  6. Describe some methods to fluorescently label a biomolecule.
  7. Describe the technique of bright field microscopy.
  8. Explain the procedure of Gram staining.
  9. List the 5 building blocks of a modern illumination apparatus.
  10. Köhler illumination, put in the correct order: [the steps of Köhler illumination shuffled]
  11. Label the microscope. What type is it? [Picture of a microscope]

I also attatched a screenshot of the questions and the PDF i learned with. I made the PDF myself, but I got lazy in the last chapter and didn’t complete it, sorry for that. In addition took a PDF with older questions and tried answering them (the greyed out parts weren’t part of the subject matter in WS20)
Questions pre-WS20.pdf (449 KB)
Summary WS20.pdf (1.79 MB)
Microscopy exam 02-02-21.png