Answer 10 out of 11 questions in 60 minutes.
- SEM vs. ESEM. Discuss advantages and disadvantages. Make a sketch.
- Explain cryotomography. Make a sketch.
- Explain high pressure freezing.
- What is a fluorophore? What properties does it have?
- Advantages of flourescence microscopy compared to other techniques?
- How can you label structures with a fluorophore?
- Explain and sketch spherical abberation and comatic abberation.
- What are the resolution limits for the eye/light microscope/electron microscope? Give examples for structures that you can view with each.
- Advantages and disadvantages when using a upright microscope on a biological sample.
- Bring Köhler illumination steps in the right order.
- Label the microscope.