- Draw a schematic setup of SEM including detectors
- Describe the different method of cryo fixation of biological samples
- What steps of preparation are necessary for cryo fixation freeze substitution
- Why does fluorescent light have a longer wavelength? How is this phenomenon called? What is the fluorescent lifetime?
- What properties do fluorescent molecules possess and what defines their color?
- Describe the principle of polarization microscopy
- Put the steps for Köhler Illumination into order
- What is the critical illumination. Discuss its advantages and disadvantages
- Label the microscope
- What is the numerical aperture? How can it be calculated and what defines it?