- How are non-conductive biological samples prepared for scanning electron microscopy (SEM)? Draw a scheme!
- Explain the two methods of cryofixation of biological samples.
- Why is there a different wavelength of emission light of the fluorophores compared to excitation wavelength, how is this phenonemon called? What is fluorescent lifetime?
- What are the intrinsic properties that characterize a fluorophore? What defines their color?
- How to estimate the thickness of ultrathin sections?
- What is fluorescence? What makes a molecule fluorescence? (something like this and one small other question about fluorescence, sorry forgot)
- Name Failure of lens (describe one of your choice)
- What image produces a simple microscope?
- What is a condenser and what is it for?
- Köhler illumination steps
- Label the microscope: inverted microscope